Fig. 5

Overexpression of KLF7 partly blocks miR-136-3p-regulated glioma cell growth, migration, and apoptosis in vitro and in vivo. a qRT-PCR analysis of KLF7 levels in LN-229 and U251 post 48 h transfection with pcDNA and pcDNA-KLF7 (or KLF7), #p < 0.01, compared with pcDNA. b Soft-agar colony formation assay analysis of glioma cell growth after 15 days transfection with miR-NC, miR-136-3p mimic, pcDNA, or pcDNA-KLF7; #p < 0.01, compared with miR-NC + pcDNA, &p < 0.01, compared with miR-136-3p + pcDNA. c Trans-well analysis of glioma cell migration after 48 h transfection with miR-NC, miR-136-3p mimic, pcDNA, or pcDNA-KLF7; #p < 0.01, compared with miR-NC + pcDNA, &p < 0.01, compared with miR-136-3p + pcDNA. d Annexin V-FITC/PI analysis of glioma cell apoptosis after 48 h transfection with miR-NC, miR-136-3p mimic, pcDNA, or pcDNA-KLF7; #p < 0.01, compared with miR-NC + pcDNA, &p < 0.01, compared with miR-136-3p + pcDNA. e, f qRT-PCR analysis of KLF7 or miR-136-3p levels after infection with lenti-viruses for 48 h in LN-229 cells, #p < 0.01, compared with lenti-vector or lenti-miR-NC. g Tumor volume after implantation of LN-229 cells in nude mice at the different time; #p < 0.01, compared with Lenti-miR-NC + Lenti-vector, &p < 0.01, compared with Lenti-miR-136-3p + Lenti-vector. h The harvested tumors after 24 days implantation of LN-229 cells. i Tumor weight of the harvested tumors from the nude mice; Lenti-miR-NC + Lenti-vector, &p < 0.01, compared with Lenti-miR-136-3p + Lenti-vector. j qRT-PCR analysis of expression of genes, including Cyclin A1, PCNA, E-cadherin, N-cadherin, Bax, and Bcl-2 in the harvested tumors; Lenti-miR-NC + Lenti-vector, &p < 0.01, compared with Lenti-miR-136-3p + Lenti-vector