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Fig. 3 | World Journal of Surgical Oncology

Fig. 3

From: Circ-FOXM1 contributes to cell proliferation, invasion, and glycolysis and represses apoptosis in melanoma by regulating miR-143-3p/FLOT2 axis

Fig. 3

Circ-FOXM1 directly targeted miR-143-3p to regulate melanoma cell proliferation, apoptosis, invasion, and glycolysis. a The binding sites between circ-FOXM1 and miR-143-3p were predicted by starBase v2.0. b, c MiR-NC or miR-143-3p in combination with WT-circ-FOXM1 or MUT-circ-FOXM1 were transfected into A2058 and A375 cells, and then the luciferase activity was determined by dual-luciferase reporter assay. d, e The levels of miR-143-3p and circ-FOXM1 in Ago2 or IgG immunoprecipitates in A2058 and A375 cells were measured by RIP assay and qRT-PCR assay. f, g A2058 and A375 cells were untransfected or transfected with si-NC, si-circ-FOXM1, pcDNA, or circ-FOXM1 and then the expression levels of circ-FOXM1 and miR-143-3p were detected by qRT-PCR. hp A2058 and A375 cells were assigned to control, si-NC, si-circ-FOXM1, si-circ-FOXM1 + anti-miR-NC, and si-circ-FOXM1 + anti-miR-143-3p groups. h The expression of miR-143-3p in A2058 and A375 cells was examined by qRT-PCR. i, j A2058 and A375 cell proliferation was evaluated by MTT assay. k A2058 and A375 cell apoptosis was analyzed by flow cytometry. l A2058 and A375 cell invasion was assessed by transwell assay. m, n The levels of glucose consumption and lactate production in A2058 and A375 cells were determined by relevant kits. o, p The protein levels of HK2 and PKM2 in A2058 and A375 cells were determined via western blot assay. *P < 0.05

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