Table 1 The nucleotide sequences of primers used for expression analysis
From: Suppressor of cytokine signaling (SOCS) genes are downregulated in breast cancer
Gene name | Primer and probe sequence | Primer and probe length | Product length |
|---|---|---|---|
HPRT1 | F: AGCCTAAGATGAGAGTTC | 18 | 88 |
R: CACAGAACTAGAACATTGATA | 21 | ||
FAM-CATCTGGAGTCCTATTGACATCGC-TAMRA | 24 | ||
SOCS1 | F: TGGCCCCTTCTGTAGGATGG | 20 | 109 |
R: GGAGGAGGAAGAGGAGGAAGG | 21 | ||
FAM-TGGCCCCTTCTGTAGGATGG-TAMRA | 20 | ||
SOCS2 | F: ACGCGAACCCTTCTCTGACC | 20 | 99 |
R: CATTCCCGGAGGGCTCAAGG | 20 | ||
FAM-CTCGGGCGGCCACCTGTCTTTGC-TAMRA | 23 | ||
SOCS3 | F: GTGGAGAGGCTGAGGGACTC | 20 | 111 |
R: GGCTGACATTCCCAGTGCTC | 20 | ||
FAM-CACCAAGCCAGCCCACAGCCAGG-TAMRA | 23 | ||
SOCS5 | F: GTGACTCGGAAGAGGATACAACC | 23 | 91 |
R: CTAACATGGGTATGGCTGTCTCC | 23 | ||
FAM-CGCTGCTTCTGCCTCCGTGACTGC-TAMRA | 24 |