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Table 3 Immunohistochemical detection methods in the original articles included in the meta-analysis by Ye et al. [14]

From: Weak HIF-1alpha expression indicates poor prognosis in resectable pancreatic ductal adenocarcinoma

Paper Patients (n) Immunohistochemistry procedures HIF-1alfa antibody source and clone Biotin based Prognostic effect of HIF-1alpha Reference
Sun et al. 2007 58 Supervision™, negative controls BA0912, Wuhan Boster, China ? High nuclear and/or cytoplasmic HIF-1alpha ➔ poor overall survival [10]
Zhu et al. 2013 63 Supervision™, negative controls Not mentioned ? High nuclear and/or cytoplasmic HIF-1alpha ➔ poor overall survival [20]
Zhang et al. 2010 65 Biotinylated goat anti-mouse or anti-rabbit (Streptavidin/peroxidase), negative controls Santa Cruz, CA, USA Yes High nuclear and/or cytoplasmic HIF-1alpha ➔ poor overall survival [18]
Miyake et al. 2008 39 Avidin-biotin (Dako LSAB™), no negative controls mentioned Novus Biologicals, Littleton, CO Yes High nuclear HIF-1alpha ➔ poor overall survival [17]
Matsuo et al. 2014 100 Avidin-biotin, no negative controls mentioned Not mentioned Yes High nuclear and/or cytoplasmic HIF-1alpha ➔ poor overall survival [9]
Ide et al. 2007 41 Biotinylated anti-mouse, anti-rabbit antibody conjugated to a peroxidase-labeled dextran polymer (Dako EnVision+™), no negative control mentioned Clone HI-67, Novus Biologicals, Littleton, CO No High nuclear and/or cytoplasmic HIF-1alpha ➔ worse disease free survival, no correlation between HIF-1alpha and overall survival [16]
Spivak-Kroizman et al. 2013 129 Bond™ Polymer Refine Detection Kit, no negative control mentioned BD Biosciences No High nuclear HIF-1alpha ➔ poor overall survival [15]
Zhao et al. 2012 95 Streptavidin biotin-peroxidase, biotinylated secondary antibody, no negative control mentioned sc-10790, Santa Cruz, CA, USA Yes HIF-1alpha localization not mentioned. High HIF-1alpha ➔ poor overall survival [19]