Table 3 Immunohistochemical detection methods in the original articles included in the meta-analysis by Ye et al. [14]
From: Weak HIF-1alpha expression indicates poor prognosis in resectable pancreatic ductal adenocarcinoma
Paper | Patients (n) | Immunohistochemistry procedures | HIF-1alfa antibody source and clone | Biotin based | Prognostic effect of HIF-1alpha | Reference |
|---|---|---|---|---|---|---|
Sun et al. 2007 | 58 | Supervision™, negative controls | BA0912, Wuhan Boster, China | ? | High nuclear and/or cytoplasmic HIF-1alpha ➔ poor overall survival | [10] |
Zhu et al. 2013 | 63 | Supervision™, negative controls | Not mentioned | ? | High nuclear and/or cytoplasmic HIF-1alpha ➔ poor overall survival | [20] |
Zhang et al. 2010 | 65 | Biotinylated goat anti-mouse or anti-rabbit (Streptavidin/peroxidase), negative controls | Santa Cruz, CA, USA | Yes | High nuclear and/or cytoplasmic HIF-1alpha âž” poor overall survival | [18] |
Miyake et al. 2008 | 39 | Avidin-biotin (Dako LSAB™), no negative controls mentioned | Novus Biologicals, Littleton, CO | Yes | High nuclear HIF-1alpha ➔ poor overall survival | [17] |
Matsuo et al. 2014 | 100 | Avidin-biotin, no negative controls mentioned | Not mentioned | Yes | High nuclear and/or cytoplasmic HIF-1alpha âž” poor overall survival | [9] |
Ide et al. 2007 | 41 | Biotinylated anti-mouse, anti-rabbit antibody conjugated to a peroxidase-labeled dextran polymer (Dako EnVision+™), no negative control mentioned | Clone HI-67, Novus Biologicals, Littleton, CO | No | High nuclear and/or cytoplasmic HIF-1alpha ➔ worse disease free survival, no correlation between HIF-1alpha and overall survival | [16] |
Spivak-Kroizman et al. 2013 | 129 | Bond™ Polymer Refine Detection Kit, no negative control mentioned | BD Biosciences | No | High nuclear HIF-1alpha ➔ poor overall survival | [15] |
Zhao et al. 2012 | 95 | Streptavidin biotin-peroxidase, biotinylated secondary antibody, no negative control mentioned | sc-10790, Santa Cruz, CA, USA | Yes | HIF-1alpha localization not mentioned. High HIF-1alpha âž” poor overall survival | [19] |