Fig. 5

Light microscope observation of steep pulse therapy for large cell lung cancer cell line L9981 in nude tumor mouse and qualitative analysis of caspase-3 protein expression with immunofluorescent staining. a Multinuclear, meganucleus, prominent nucleoli, and relatively more mitoses were noticed in control group (× 400). b A homogeneous red-stained patch was noticed in the anode region, with blurred cell structure, and appeared with coagulation necrosis (× 400). c Cell structures of cathode were still clear with no significant changes, so as the deep subcutaneous tumor cells (× 400). d Tumor cell necrosis, darkening of cytoplasm eosin staining, dark staining of karyopyknosis, cytoplasm eosin staining, and nuclear pyknosis hyperchromatic (× 400). e Necrosis border was clear, with karyopyknosis, karyorrhexis, and karyolysis (× 400). f Tumor tissue necrosis, obviously, there are large necrosis area (× 400). g Karyopyknosis, karyorrhexis, and karyolysis (× 400). h Yellow fluorescence spotty distribution of caspase-3 by fluorescence microscopy. i Experimental group caspase-3 expression by fluorescence microscopy was obviously more than the control group