Two different protein expression profiles of oral squamous cell carcinoma analyzed by immunoprecipitation high-performance liquid chromatography

Kim, Soung Min; Jeong, Dasul; Kim, Min Keun; Lee, Sang Shin; Lee, Suk Keun

doi:10.1186/s12957-017-1213-5

Table 2 Antibodies used in this study

From: Two different protein expression profiles of oral squamous cell carcinoma analyzed by immunoprecipitation high-performance liquid chromatography

Signaling proteins	Number	Antibodies
Cytoskeletal proteins	1	α-Tubulin^a
Growth factor-related proteins	5	EGFR^b, c-erbB2^b, TGF-β1^d, bFGF^a, HGF^b
Proliferation-related proteins	9	eIF5A^c, DHS^c, DOHH^c, PCNA^c, MPM-2^b, CDK4^a, cMyc^a, MAX^a, hTERT^a
Transcription signaling proteins	3	NFkB^b, p38^a, E2F-1^a
Apoptosis-related proteins	14	FAS^a, FASL^a, PARP^a, BAX^a, NOXA^a, PUMA^a, BAD^a, BAK^a, BID^a, caspase 3^a, caspase 8^a, caspase 9^a, FADD^a, FLIP^a,
Cell survival-related proteins	3	pAKT^c, MDM2^a, BCL2^a
Tumor suppressor proteins	8	p16^a, p21^a, p53^a, p63^a, RB1^a, PTEN^a, PTCH^a, NF-1^b
Oncoproteins	9	14-3-3^a, CEA^c, STAT3^a, survivin^d, DMBT1^a, maspin^a, snail^a, KRAS^c, PIM1^a
Protection proteins	5	HO-1^a, caveolin^a, HSP-70^a, FAK^a, TGase-1^b
Proinflammation proteins	2	TNFα, SHP-1
WNT/β-catenin pathway proteins	4	SHH^a, β-catenin^b, WNT1^a, APC^a
Matrix proteolysis proteins	4	MMP-1^c, MMP-2^c, MMP-9^a, elaffin^a
Angiogenesis-related proteins	5	HIF^d, VEGF^d, vWF^c, angiogenin^a , CMG2^b
Total	72

Abbreviation: pAKT v-akt murine thymoma viral oncogene homolog (phosphorylated at Thr 308), APC adenomatous polyposis coli, BAD BCL2 associated death promoter, BAK BCL2 antagonist/killer, BAX BCL2 associated X, BCL-2 B-cell leukemia/lymphoma-2, BID BH3 interacting-domain death agonist, CDK4 cyclin dependent kinase 4, CEA carcinoembryonic antigen, CMG2 capillary morphogenesis protein 2, DHS deoxyhypusine synthase, DOHH deoxyhypusine hydroxylase, DMBT1 deleted in malignant brain tumors 1, E2F-1 transcription factor, EGFR epithelial growth factor receptor, eIF5A eukaryotic translation initiation factor 5A, FADD FAS associated via death domain, FAK focal adhesion kinase, FAS CD95/Apo1, FASL FAS ligand, bFGF basic fibroblast growth factor, FLIP FLICE-like inhibitory protein, HGF hepatocyte growth factor, HIF hypoxia inducible factor, HO-1 hemoxygenase 1, HSP-70 heat shock protein-70, KRAS V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog, MAX myc-associated factor X, MDM2 mouse double minute 2 homolog, MMP-1 matrix metalloprotease-1, MPM-2 mitotic protein monoclonal 2, cMyc V-myc myelocytomatosis viral oncogene homolog (avian), NF-1 neurofibromin-1, NFkB nuclear factor kappa-light-chain-enhancer of activated B cells; NOXA phorbol-12-myristate-13-acetate-induced protein 1; PARP poly-ADP ribose polymerase, PCNA proliferating cell nuclear antigen, PIM1 pivotal integration site 1, PTCH patched homolog, PTEN phosphatase and tensin homolog, PUMA p53 up-regulated modulator of apoptosis, RB1 retinoblastoma 1, SHH sonic hedgehog, SHP-1 short helical protein-1, SOS-1 Son of sevenless-1, STAT3 signal transducer and activator of transcription-3, hTERT human telomerase reverse transcriptase, TGase-1 transglutaminase-1, TGF-β1 transforming growth factor-β1, TNFα tumor necrosis factor-α, VEGF vascular endothelial growth factor, vWF von Willebrand factor
^aSanta Cruz Biotechnology, USA
^bDAKO, Denmark
^cNeomarkers, CA, USA
^dZYMED, CA, USA

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ISSN: 1477-7819

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