Fig. 5

RBM5 inactivated Wnt/β-catenin signaling and induced apoptosis in SHG44 cells. Proteins were extracted from SHG44 cells with or without RBM5 overexpression. a Expression of β-catenin, P-GSK-3β, Cyclin D1, DKK1, Bax, Bcl-2, cleaved caspase3, and TNF-α were determined by western blot. GAPDH was used internal control. b FACS analysis of the apoptotic rate of SHG44 cells without RBM5 overexpression. c FACS analysis of the apoptotic rate of SHG44 cells with RBM5 overexpression. All experiments were repeated in triplicates. *P < 0.05 showed the significant difference