Fig. 2

RBM5 was successfully overexpressed in U251 and SHG44 cells. RBM5 was introduced into U251 and SHG44 cells by lentivirus and confirmed by RT-qPCR and western blot analysis. a RT-qPCR analysis of RBM5 mRNA in U251 cells. b RT-qPCR analysis of RBM5 mRNA in SHG44 cells. c Western blot analysis of RBM5 protein in U251 and SHG44 cells. Data were shown as means ± SD of three independent experiments. *P < 0.05 was considered as statistically significant