Table 1 Primers and restriction enzyme used for analysis of these three SNPS
From: Common genetic polymorphisms in pre-microRNAs and risk of bladder cancer
SNPs | Primers (5′–3′) | Products (bp) | Restriction enzyme |
|---|---|---|---|
hsa-mir-196a2 rs11614913 C/T | F: CCCCTTCCCTTCTCCTCCAGATA | 149 | MspI |
R: CGAAAACCGACTGATGTAACTCCG | |||
hsa-mir-499 rs3746444 A/G | F: CAAAGTCTTCACTTCCCTGCCA | 146 | BclI |
R: GATGTTTAACTCCTCTCCACGTGATC | |||
hsa-mir-146a rs2910164 C/G | F: CATGGGTTGTGTCAGTGTCAGAGCT | 147 | SacI |
R: TGCCTTCTGTCTCCAGTCTTCCAA |