Table 2 Antibodies used for immunohistochemical study
From: Prolactin-producing pituitary adenoma with atypical spindle cell morphology: a case report
Primary antibody | Source | Pretreatment | Category | Dilution |
|---|---|---|---|---|
PRL | DAKO | None | Rabbit polyclonal | 1:1500 |
GH | DAKO | None | Rabbit polyclonal | 1:1500 |
FSH | DAKO | None | Rabbit polyclonal | 1:100 |
ACTH | DAKO | None | Mouse monoclonal | 1:2500 |
Cytokeratin | Leica BOND | Proteinase K | Mouse monoclonal | 1:100 |
EMA | DAKO | Tris/EDTA pH 8 | Mouse monoclonal | 1:100 |
S-100 protein | Nichirei | None | Rabbit polyclonal | Predilute |
Vimentin | Leica BOND | Tris/EDTA pH 8 | Mouse monoclonal | 1:200 |
GFAP | Leica BOND | Tris/EDTA pH 8 | Mouse monoclonal | 1:300 |
Synaptophysin | Leica BOND | Tris/EDTA pH 8 | Mouse monoclonal | 1:100 |
Chromogranin-A | DAKO | Tris/EDTA pH 8 | Mouse monoclonal | 1:400 |
bcl-2 | DAKO | Tris/EDTA pH 8 | Mouse monoclonal | 1:1000 |
TP53 | Leica BOND | Tris/EDTA pH 8 | Mouse monoclonal | 1:40 |
Ki-67 | DAKO | Tris/EDTA pH 8 | Mouse monoclonal | 1:400 |