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Table 2 Antibodies used for immunohistochemical study

From: Prolactin-producing pituitary adenoma with atypical spindle cell morphology: a case report

Primary antibody Source Pretreatment Category Dilution
PRL DAKO None Rabbit polyclonal 1:1500
GH DAKO None Rabbit polyclonal 1:1500
FSH DAKO None Rabbit polyclonal 1:100
ACTH DAKO None Mouse monoclonal 1:2500
Cytokeratin Leica BOND Proteinase K Mouse monoclonal 1:100
EMA DAKO Tris/EDTA pH 8 Mouse monoclonal 1:100
S-100 protein Nichirei None Rabbit polyclonal Predilute
Vimentin Leica BOND Tris/EDTA pH 8 Mouse monoclonal 1:200
GFAP Leica BOND Tris/EDTA pH 8 Mouse monoclonal 1:300
Synaptophysin Leica BOND Tris/EDTA pH 8 Mouse monoclonal 1:100
Chromogranin-A DAKO Tris/EDTA pH 8 Mouse monoclonal 1:400
bcl-2 DAKO Tris/EDTA pH 8 Mouse monoclonal 1:1000
TP53 Leica BOND Tris/EDTA pH 8 Mouse monoclonal 1:40
Ki-67 DAKO Tris/EDTA pH 8 Mouse monoclonal 1:400