Figure 3
Effects of Gem on the expression of Bcl-2 and Bax in BXPC-3. (A) Cells were treated with 0–25 uM gemcitabine for 72 h before collecting proteins. (B) BXPC-3 cells were treated with 25 μM PD98059 for 24 h, then treated with 0–25 μM gemcitabine for 72 h. Cell lysates were subjected to Western blot analysis and incubated with anti-pERK1/2, anti-Bax, anti-Bcl-2, or anti-β-actin monoclonal antibodies. The gel image analysis software was used to analyze the absorbance values of Bcl-2 and Bax.