Figure 4

CCL25-induced collagenase expression by ovarian cancer cells. Cells were tested for their ability to express collagenases (MMP-1, MMP-8, and MMP-13) mRNA and protein. OVCAR-3 and CAOV-3 cells were cultured for 24 hours alone, with 100 ng/mL of CCL25, or CCL25 + 1 μg/mL of mouse anti-CCR9 antibody. Total RNA was isolated and quantitative RT-PCR analysis was performed for mRNA expression of collagenases (upper panel) and transcript copies are presented relative to actual copies of 18 S rRNA. Active collagenases were quantified by Fluorokine and Biotrak assays in conditioned media (lower panel). MMP expression below the detectable limit of the RT-PCR is designated as below detection (BD). Asterisk(s) indicate statistical differences (p < 0.01) between untreated and CCL25-treated OvCa cells.