Figure 2
From: P38 MAP Kinase inhibition promotes primary tumour growth via VEGF independent mechanism
Serum VEGF levels for control and SB-202190 treated animals. Animals (n = 10, per group) were randomised to receive control (vehicle) or SB-202190 (2.5 μM/kg) via an intraperitoneal route on a daily basis. After 24 days animals were sacrificed and blood was collected by intracardiac puncture, left to stand overnight prior to centrifugation for 20 minutes at 10,000 rpm. Serum was collected and stored at -80°C for determination of VEGF concentrations using commercially available ELISA kits according to the manufacturer's instructions.