Table 1 Conditions of MS-PCR
| Â | Denaturation | Annealing | Extension | ||||
|---|---|---|---|---|---|---|---|
|  | Temp(°C) | Time(min) | Temp (°C) U/M | Time(sec) | Cycles | Temp (°C) | Time(min) |
p14ARF | 95 | 5 | 62/62 | 30 | 40 | 72 | 7 |
p16INK4a | 95 | 5 | 63/63 | 30 | 40 | 72 | 7 |
E-cadherin | 95 | 5 | 55/57 | 30 | 40 | 72 | 7 |
MINT1(M1) | 95 | 5 | 52/52 | 30 | 37 | 72 | 7 |
MINT2(M2) | 95 | 5 | 59/59 | 30 | 40 | 72 | 7 |
MINT31(M31) | 95 | 5 | 62/60 | 30 | 38 | 72 | 7 |