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Table 1 Conditions of MS-PCR

From: The combination effect of sodium butyrate and 5-Aza-2'-deoxycytidine on radiosensitivity in RKO colorectal cancer and MCF-7 breast cancer cell lines

  Denaturation Annealing Extension
  Temp(°C) Time(min) Temp (°C) U/M Time(sec) Cycles Temp (°C) Time(min)
p14ARF 95 5 62/62 30 40 72 7
p16INK4a 95 5 63/63 30 40 72 7
E-cadherin 95 5 55/57 30 40 72 7
MINT1(M1) 95 5 52/52 30 37 72 7
MINT2(M2) 95 5 59/59 30 40 72 7
MINT31(M31) 95 5 62/60 30 38 72 7