Table 1 Primer sequences and annealing temperatures for PCR
Primer set | Position | Annealing temperature |
|---|---|---|
β-catenin exon3 | ||
F:GTC TGA GGA GCA GCT TCA GT | 77–96 | 56°C |
R:CAT TAG TGG GAT GAG CAG CA | 559–578 | |
Axin | ||
F1:GCG GGA CAG ATT GAT TCA CT | 28–47 | 54°C |
R1:TCG GCA GGT ATC CAG ATA TG | 811–830 | |
F2:TCT GTA GTG ACC AGA GCT CT | 771–790 | 54°C |
R2:GAC GAT GGA TCG CCG TCC T | 1380–1398 | |
F3:AGT TCG CGG AGG AGC TCA T | 1272–1290 | 54°C |
R3:CCT CAA TGA TCC ACT GCA TG | 2011–2030 | |
F4:AGG ATG CGG AGA AGA ACC AG | 1986–2005 | 54°C |
R4:TCC TGA GTA CGA GGT CAT CT | 2770–2789 |