Figure 1

A) p21 expression in adjacent tumor tissue. Nuclear immunostaining is evident (original magnification, 100×). B) HIF-1α expression in invading regions of tumor. Variability in intensity of nuclear immunostaining is evident, accompanied by cytoplasmic staining (original magnification, 100×). C) Nuclear immunostaining of p53 in tumor tissue (original magnification, 100×). D) Tumor tissue stained with H&E (original magnification, 200×). Tumor tissue contains cells (arrow) with characteristic features of apoptosis.