Figure 3

Cisplatin (DDP) treatment induces sCLU and pERK1/2 up-regulation. Human OS lines KH OS, Sa OS, and U-2 OS were treated with increasing concentrations of DDP (0 to 10 μg/mL) for 72 hours. Western blot analysis was done to determine expression of clusterin and pERK1/2 in indicated OS cell lines. The membranes were stripped and reprobed with anti-β-actin antibody to ensure even loading of proteins in each lane. Results shown are from representative experiments repeated at least twice with similar findings.