Figure 3

NCTD inhibits Raf/MEK/ERK signaling pathway in U87 (A) and C6 (B) cells. Cells were treated with the indicated concentrations of NCTD for 24 h. After treatment, whole cell protein extracts were prepared, and equal amounts of total protein were resolved on SDS-PAGE gels. Western blotting analysis was performed using specific antibodies against the indicated proteins.