Cortactin expression in HCCs (400× magnification). (A) Bile duct epithelial cells (thin arrow) were strong positive, whereas HCC tissue (thick arrow) was negative. (B) Vascular endothelial cells (arrowhead) were positive with HCC tissue of weak positive expression. (C) HCC tissue showed strong positivity with diffuse staining of cytoplasm. (D) HCC tissue showed strong positive. Cancer cells were in a state of chaos with severe morphosis. Cancer embolus (double tail arrow) in portal vein can be seen.