Figure 3

Effect of DACT2 knockdown on cell cycle distribution of HCC cell line. (A) Real-time PCR analysis showing expression of DACT2 in MHCC97L cell was significantly reduced after treatment with DACT2 siRNA compared with negative siRNA (*P < 0.01, Student’s t- test). (B) Cell cycle distribution of MHCC97L cell treated with DACT2 siRNA (lower) or negative control (upper). (C) The alteration of cell cycle distribution was different significantly (*P < 0.01, Student’s t test). All assays were performed in triplicate wells.