Table 2 Pathological and immunohistochemical findings in duodenal GISTs, and a list of primary antibodies used in diagnosis
Variable | Dilution, Source of Antibodies | Total | SkF group | non-SkF group | P value |
|---|---|---|---|---|---|
Number | 20 | 12 | 8 | ||
Tumor size – mm | 46.5 ± 63.4 | 23.3 ± 8.2 | 81.2 ± 92.2 | <0.001 | |
Spindle type cell - no. (%) | 13 (65%) | 8 (67%) | 5 (63%) | 0.608 | |
Necrosis - no. (%) | 6 (30%) | 0 (0%) | 6 (75%) | <0.001 | |
Ulceration - no. (%) | 15 (75%) | 7 (58%) | 8 (100%) | 0.051 | |
Mitosis - no. per 50 HPF | 89.6 ± 233.3 | 0.0 ± 0.0 | 224.0 ± 336.6 | <0.001 | |
Mib-1 index -% | 1:200, autoclave; MBL | 11.6 ± 17.4 | 1.8 ± 1.2 | 26.3 ± 20.3 | <0.001 |
KIT - no. (%) | 1:100, autoclave; Dako | 20 (100%) | 12 (100%) | 8 (100%) | 0.999 |
CD34 - no. (%) | 1:100, autoclave; Ventana | 14 (70%) | 9 (75%) | 5 (63%) | 0.455 |
S-100 - no. (%) | 1:1000; Dako | 14 (70%) | 9 (75%) | 5 (63%) | 0.455 |
α-smooth-muscle actin - no. (%) | 1:200, autoclave; Dako | 14 (70%) | 9 (75%) | 5 (63%) | 0.455 |
Desmin - no. (%) | 1:40, autoclave; Dako | 11 (55%) | 8 (67%) | 3 (38%) | 0.205 |