Figure 3

A. Representative SSCP* analysis of B-raf gene, exon 15 of seven colorectal cancer cells. Lanes 1, 3-7: tumor samples with normal band pattern (black arrows). Lane 2: tumor sample with the typical band pattern of a V600E mutation (arrow heads). *SSCP: Single strand conformation polymorphism. B. Sanger heterozygous analysis showing a heterozygous B-raf mutation (exon 15) at codon 600 (p.V600E, c.1799T > A).