Figure 4

Amiloride inhibited MKN45 derived-tumor growth. We intraperitoneally injected 5 × 10⁶ MKN45 cells into 4- to 5-week-old male BALB/c nude mice. The mice were randomly divided into two groups of twelve per group; 14 days after MKN45 implantation, the treatment group received the first dose of amiloride dissolved in saline solution. Dosage and administration schedules were based on our preliminary toxicologic and pharmacokinetic studies; amiloride was given orally to tumor-bearing mice at 50 mg/kg every day at the first three days of one week for a total time of four weeks. In parallel, the control group received saline solution. Mice were sacrificed when they became moribund, and the sacrifice date was recorded to calculate the survival time. (A) Amiloride inhibited MKN45-derived tumor growth. Con, control; Amiloride, group treated with 50 mg/kg amiloride.