Figure 5
From: RNAa-mediated overexpression of WT1 induces apoptosis in HepG2 cells
dsWT1-319 treatment induced time-dependent apoptosis in HepG2 cells detected by flow cytometry using a double-staining method with FITC-conjugated annexin V and PI. Annexin V-stained cells indicates the early apoptotic cells, whereas Annexin V + propidium iodide-stained cells are the late apoptotic cells. A representative blot is shown from three independent experiments with identical results.