Fig. 1

Characterization of the expressions and localizations of CD8 and CD103 in human HCC and ICC tissues by mIHC. An enlarged subsection of the core was highlighted, showing each of the individual markers in the composite image after spectral unmixing, together with the DAPI nuclear marker (pseudo-colored blue) and the autofluorescence signal (pseudo-colored black). A The expressions of CD8 (membrane, pseudo-colored red) and CD103 (membrane, pseudo-colored brown) in adjacent normal tissues of HCC identified by CK (membrane, pseudo-colored green), together with the DAPI nuclear marker (pseudo-colored blue). B Expressions of CD8 and CD103 in tumor tissues of HCC. C Expressions of CD8 and CD103 in adjacent normal tissues of ICC. D Expressions of CD8 and CD103 in tumor tissues of ICC. Scale bar for the first image of A, B, C, or D, 200 μm