Fig. 2

Microscopic images of the PSC. a The tumor demonstrated a spindle cell-like morphology with poor differentiation (× 200). b The tumor cells showed a positive nuclear signal for TTF-1 (× 200). c The tumor cells showed a weakly positive cytoplasmic signal for PCK (× 200). d The tumor cells showed a weakly positive cytoplasmic signal for EMA (× 200). e The tumor cells showed a negative cytoplasmic signal for P40 (× 200). f PD-L1 TPS immunohistochemistry analysis showed at least 60% (× 200)