Fig. 5

MiR-1827 directly targeted SLC1A5. A The sequences of SLC1A5 3′UTR WT/MUT vectors were shown. B–C Dual-luciferase reporter assay was used to verify the interaction between SLC1A5 and miR-1827. D The SLC1A5 mRNA expression in NSCLC tumor tissues and adjacent normal tissues was measured by qRT-PCR. E IHC staining was used to determine the SLC1A5-positive cells in NSCLC tumor tissues and adjacent normal tissues. F The SLC1A5 protein expression in NSCLC tumor tissues and adjacent normal tissues was analyzed using WB analysis. G WB analysis was performed to test the SLC1A5 protein expression in NSCLC cells (A549 and NCI-H1299) and BEAS-2B cells. H–J The correlations among circ_0000808, miR-1827, and SLC1A5 expression in NSCLC tumor tissues were analyzed by Pearson correlation analysis. **P < 0.01, ***P < 0.001