Fig. 2

Construction of immune-related gene signatures and identification of immune gene subtypes. a Venn diagram presented 1428 overlapping DEGs among three immune clusters was identified. b Functional annotations of DEGs based on GO analysis and the circle size mean the number of genes enriched. c Functional annotations of DEGs based on KEGG pathway analysis and the circle size represent the enriched gene number. d Unsupervised clustering of the immune-related gene signatures in the combined CM cohort. The immune cluster, gene cluster, survival status, and ages served as patient annotations. Red means high expression levels, and blue represents low expression levels of these genes. e Survival analysis of distinct gene clusters in the combined CM cohort. K-M curves with P < 0.001 suggested that the difference in survival was significant across the three clusters. f Variations in the abundance of infiltrating immune cells across gene clusters A, B, and C using “CIBERSORT” analysis. The value of Y-axis is standard score. “*” represents the statistical P-value (*P < 0.05; **P < 0.01; ***P < 0.001). g Differences in the abundance of infiltrating immune cells among gene clusters A, B, and C using “ssGSEA” analysis. “*” represents the statistical P-value (*P < 0.05; **P < 0.01; ***P < 0.001). h The expression of genes related to immune checkpoints in three gene clusters