Table 1 The sequence of RT-qPCR primers
Gene name | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
TNF | ACTTTGGAGTGATCGGCCCC | TCGGGGTTCGAGAAGATGAT |
Arg1 | ACTTAAAGAACAAGAGTGTGATGTG | TCCAATTGCCAAACTGTGGTC |
IL-10 | GGCACCCAGTCTGAGAACAG | GGCAACCCAGGTAACCCTTA |
TGF-β | GGAAATTGAGGGCTTTCGCC | CCGGTAGTGAACCCGTTGAT |
GAPDH | ACGGATTTGGTCGTATTGGG | CGCTCCTGGAAGATGGTGAT |
miR-21 | CGGCGGTAGCTTATCAGACTGATGT | GTGCAGGGTCCGAGGT |
miR-8485 | CACACACACACACACACGUAU | ATACGTGTGTGTGTGTGTGTG |
miR-3691-5p | AGUGGAUGAUGGAGACUCGGUAC | GTACCGAGTCTCCATCATCCACT |
miR-4465 | CUCAAGUAGUCUGACCAGGGGA | TCCCCTGGTCAGACTACTTGAG |
miR-575 | GAGCCAGUUGGACAGGAGC | GCTCCTGTCCAACTGGCTC |
miR-320c | AAAAGCUGGGUUGAGAGGGU | ACCCTCTCAACCCAGCTTTT |
miR-4761-5p | ACAAGGUGUGCAUGCCUGACC | GGTCAGGCATGCACACCTTGT |
miR-19a-3p | AGUUUUGCAUAGUUGCACUACA | TGTAGTGCAACTATGCAAAACT |
miR-19b-3p | UGUGCAAAUCCAUGCAAAACUGA | TCAGTTTTGCATGGATTTGCACA |
miR-1285-3p | UCUGGGCAACAAAGUGAGACCU | AGGTCTCACTTTGTTGCCCAGA |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |