Fig. 3

Exosomes promote macrophage M2 polarization through the PI3K/AKT signaling pathway and accelerate the proliferation of lung cancer cells. H1299 cells were cultured alone or co-cultured with the macrophage stimulated with PBS or exosome secreted from normoxia or hypoxia H1299 cells. A) Western blot was performed to detect the expression of PI3K/AKT signaling pathway associated genes. B H1299 proliferation level was analyzed through CCK8 assay and C clone formation assay. Data were expressed as mean ± SD. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001