Fig. 4

RNF180 interacted with c-Myc and enhanced its ubiquitination in NSCLC cells. A Functional analysis indicated that RNF180 negatively correlated with C-myc. B qRT-PCR was used to examine the relative mRNA levels of C-myc in oeNC and oeRNF180 transfecting H358 and H1975 cells. C Western blot was used to examine the protein levels of C-myc in oeNC or oeRNF180 transfecting cells with or without the treatment of the proteasome inhibitor MG132. D CO-IP assay indicated that RNF180 interacted with C-myc. E Overexpression of RNF180 enhanced the ubiquitination of C-myc in NSCLC cells