Fig. 2

Expression of E2F-1 and ATM in transfected DDP-resistant NPC cells. Note: A qRT–PCR was used to determine the relative mRNA expression of E2F-1 and ATM in DDP-resistant NPC cells (CNE2/DDP and HNE1/DDP); B, C western blotting was used to determine the protein expression of E2F-1 and ATM in CNE2/DDP cells (B) and HNE1/DDP cells (C). The experiment was repeated independently three times. Comparisons among multiple groups were analyzed using one-way ANOVA, while intergroup differences were tested by Tukey’s HSD test. *, P < 0.05 compared with the blank group and control shRNA group; #, P < 0.05 compared with the E2F-1 shRNA#1 group and E2F-1 shRNA#2 group; &, P < 0.05 compared with the ATM group