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Fig. 2 | World Journal of Surgical Oncology

Fig. 2

From: Integrated analysis of ceRNA network reveals potential prognostic Hint1-related lncRNAs involved in hepatocellular carcinoma progression

Fig. 2

Microarray profiling and validation in Hint1-deficient Huh7 cells. A mRNA level of Hint1 in both siCTRL- and siHINT1-treated Huh7 cells determined by qRT -PCR (normalized to the mRNA level of β-ACTIN, data was presented as mean ± SEM; n = 3). B Protein level of HINT1 and β-ACTIN in both siCTRL- and siHINT1-treated Huh7 cells determined by western blot. Duplicate lncRNAs (C) and mRNAs (D) expression microarray analysis of Huh7 cells treated with either siCTRL or siHINT1 were subjected to log2-transformed gene expression followed by K-means clustering (fold-change (FC) ≥ 2, while false discovery rate (FDR) ≤ 0.05, up- and downregulated lncRNAs and mRNAs are indicated in red and green, respectively). E qRT-PCR was performed on twelve lncRNAs and mRNAs with the highest levels of elevation and decrease in the microarray analysis and normalized to mRNA level of β-ACTIN (FCs were computed in terms of percentage fold change of the siHINT1-treated transcriptome relative to the siCTRL-treated transcriptome, data was presented as mean ± SEM; n = 3); Mini heat map representing the selected twelve lncRNA (F) and mRNA (G) in microarray analysis. ***P ≤ 0.001

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