Fig. 6

miR-495-3p exerted its biological function by targeting HMGB1 in vivo and in vitro. a HMGB1 protein levels were restored after co-transfection with miR-495-3p mimics and HMGB1 plasmid in HT29 and SW480 cells. B Overexpression of HMGB1 attenuated the effect of miR-495-3p on the proliferation of CRC cells. C, D Overexpression of HMGB1 attenuated the effect of miR-495-3p on the migration of CRC cells. E, F Representative images of implanted mice and dissected tumour tissues. The HMGB1 lentivirus group with the largest tumour volume was placed at the front, and the miR-495-3p lentivirus group with the smallest tumour volume was placed at the end for the sake of facilitating the observation of tumour size. G Growth curves of the tumours. Tumour volumes were measured using a slide calliper every 4 days (n = 4). H Weights of xenograft tumour in different groups (n = 4). J HMGB1 protein levels in xenograft tumour tissues were detected by Western blot analysis. *p < 0.05, **p < 0.01 and ***p < 0.001