Fig. 5From: Hsa_circ_0020850 promotes the malignant behaviors of lung adenocarcinoma by regulating miR-326/BECN1 axisBECN1 was a functional gene of miR-326. A The binding regions between BECN1 and miR-326 were displayed. B–E Dual-luciferase reporter and RIP assays were conducted to analyze the association between BECN1 and miR-326. F The mRNA expression of BECN1 was determined by RT-qPCR in lung adenocarcinoma tissues and control. G The correlation between BECN1 and miR-326 was conducted by Pearson’s correlation analysis. H, I Western blot assay was used to examine BECN1 level in lung adenocarcinoma tissues and cells along with matched controls. J After transfection with in-miR-con or in-miR-326, the protein expression of BECN1 was assessed by western blot assay in A549 and HCC827 cells. *P < 0.05Back to article page