Fig. 4

Knockdown of circ_0020850-induced effects on lung adenocarcinoma cells were abolished by inhibition of miR-326. A A549 and HCC827 cells were transfected with in-miR-326 to inhibit miR-326 expression. B–M A549 and HCC827 cells were transfected with si-con, si-circ_0020850, si-circ_0020850+in-miR-con, or si-circ_0020850+in-miR-326. B, C The expression level of miR-326 was analyzed by RT-qPCR. D–G The migration and invasion were assessed by wound healing and transwell assays, respectively. H, I The proliferation ability of A549 and HCC827 cells was analyzed by colony-forming assay. J, K The matrigel angiogenesis assay was conducted in A549 and HCC827 cells post-transfection. L, M The flow cytometry assay was carried out to assess apoptosis rate in A549 and HCC827 cells. *P < 0.05