Fig. 6

A Western blot assay of TMED2, TLR4, NF-κB-p-p65, IL-1β, and IL-18 expression in the Con, sh-TMED2, control-shTMED2, and Spa. + sh-TMED2 groups in vitro. B Quantification of TMED2, TLR4, NF-κB-p-p65, IL-1β, and IL-18 protein expression. C Western blot assay of TMED2, TLR4, NF-κB-p-p65, IL-1β, and IL-18 expression in vivo. D Quantification of TMED2, TLR4, NF-κB-p-p65, IL-1β, and IL-18 protein expression. Protein levels were normalized to β-actin. (sh-TMED2 vs. Con group, *P < 0.05; Spa. + sh-TMED2 vs. sh-TMED2 group, #P < 0.05, n = 6 per group)