Fig. 4

A Tumor volume in the Con, sh-TMED2, and control-shTMED2 mice after 28 days. B IHC for TMED2 and determination of the Ki-67 index. C TUNEL-DAPI staining assay (× 400) and D quantification of TUNEL(+) cells. E Western blot assay of CEA, NSE, and EGFR expression in vivo. F Quantification of TMED2, CEA, NSE, and EGFR expression. Protein levels were normalized to β-actin. (sh-TMED2 vs. other groups, *P < 0.05, n = 6 per group)