Fig. 4

Silencing of circ_0047921-mediated effects on proliferation, migration, invasion, EMT and glycolysis of lung cancer cells could be reversed by knockdown of miR-1287-5p. A The expression of miR-1287-5p was assessed with RT-qPCR in A549 and H1299 cells transfected with inhibitor-NC or miR-1287-5p inhibitor. B–J A549 and H1299 cells were transfected with si-NC, si-circ_0047921, si-circ_0047921 + inhibitor-NC, or si-circ_0047921 + miR-1287-5p inhibitor. B, C The cell viability was assessed with CCK8 assay. D Edu assay was performed in A549 and H1299 cells. E, F Transwell assay was used to analyze migration and invasion abilities of transfected A549 and H1299 cells. G The western blot assay was applied to test E-cadherin, Vimentin, and N-cadherin expression. H, I Glucose consumption and lactate production of transfected A549 and H1299 cells were displayed. J Expression of LDHA, HK2, and GLUT1 was assessed by western blot assay in A549 and H1299 cells. *P < 0.05