Fig. 6
From: miRNA-214-5p inhibits prostate cancer cell proliferation by targeting SOX4
Specific knockdown of SOX4 significantly inhibits cell proliferation. Lentivirus-packaged SOX4 shRNA and control shRNA were transduced into DU-145 and PC-3 cells, respectively. The knockdown efficiency of shRNA at transcription and translation levels was verified by RT-qPCR (A) and Western blot (B). CCK-8 kit was used to detect the proliferation of SOX4 shRNA and control shRNA cell lines (C, D) at 6 h, 24 h, 48 h, and 72 h, respectively. GAPDH is an internal reference protein. miRNA control group was used as control (*P < 0.05, **P < 0.01)