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Fig. 6 | World Journal of Surgical Oncology

Fig. 6

From: Circular RNA circVAPA contributes to non-small-cell lung cancer progression via miR-342-3p-dependent regulation of ZEB2

Fig. 6

MiR-342-3p interacts with the 3′UTR of ZEB2 in NSCLC cells. A Binding regions between miR-342-3p and 3′UTR of ZEB2 were shown. B Dual-luciferase reporter assay was performed to show the luciferase activity in A549 and H1299 cells. C After transfection with miR-342-3p or miR-NC, the expression of ZEB2 was assessed in A549 and H1299 cells. D The expression of ZEB2 was measured by RT-qPCR assay in NSCLC tissues along with adjacent normal tissues. E–F The correlation relationship between ZEB2 and circVAPA or miR-342-3p was analyzed by Pearson’s correlation analysis. G The protein expression of ZEB2 was analyzed by western blot assay in A549 and H1299 cells. H–P A549 and H1299 cells were transfected with miR-NC, miR-342-3p, miR-342-3p + vector, or miR-342-3p + ZEB2. H–J The cell proliferation was determined by MTT and colony-forming assays. K The flow cytometry assay was conducted to evaluate the apoptosis rate of A549 and H1299 cells. L, M Western blot assay was conducted to measure the expression of proliferation-associated protein (Ki-67) and pro-apoptotic protein Bax. N, O The migration and invasion capabilities were examined by transwell analysis. P The expression levels of E-cadherin, N-cadherin, and Vimentin were examined by western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001

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