Fig. 5

CircVAPA silencing-mediated anti-tumor effects are largely overturned by the interference of miR-342-3p in NSCLC cells. A The RT-qPCR assay was performed for examining the expression of miR-342-3p in NSCLC cells and control. B The knockdown efficiency of miR-342-3p was confirmed by RT-qPCR assay in A549 and H1299 cells. C–J A549 and H1299 cells were transfected with si-NC, sh-circVAPA#1, sh-circVAPA#1 + anti-NC, or sh-circVAPA#1 + anti-miR-342-3p. C–E MTT and colony-forming assays were performed for examining the cell proliferation of A549 and H1299 cells. F The apoptotic cells were determined by flow cytometry assay. G Western blot assay was conducted to measure the expression of Ki-67 and Bax. (H-I) The transwell assay was conducted to evaluate the migration and invasion of A549 and H1299 cells. J The expression levels of E-cadherin, N-cadherin, and Vimentin were evaluated by western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001