Fig. 4

MiR-342-3p was a direct target of circVAPA. A Cytoplasmic and nuclear fraction RNAs were isolated, circVAPA expression was determined by RT-qPCR assay. B Schematic illustration showed the overlap of the target miRNAs of circVAPA predicted by circBANK and Starbase. C The expression levels of miR-342-3p and miR-132-3p were examined by RT-qPCR assay in A549 and H1299 cells transfected with sh-NC, or sh-circVAPA#1. D The expression of miR-342-3p was assessed by RT-qPCR assay in NSCLC tissues and neighboring normal tissues. E Pearson’s correlation analysis was used to analyze correlation between miR-342-3p and circVAPA. F Binding regions between miR-342-3p and circVAPA were shown. G The transfection efficiency of miR-342-3p was checked by RT-qPCR assay. H The relative luciferase activity was analyzed in A549 and H1299 cells. I–K The interaction association between miR-342-3p and circVAPA was confirmed by RNA pull-down and RIP assays. ***P < 0.001