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Fig. 4 | World Journal of Surgical Oncology

Fig. 4

From: LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis

Fig. 4

The target relationship between miR-485-5p and MYO6 in CRC cells. A The target sites of miR-485-5p and MYO6. B Luciferase activity of MYO6-3′UTR-WT and MYO6-3′UTR-MUT vectors was detected in LoVo and SW620 cells co-transfected with miR-485-5p mimic or miR-NC. CE MYO6 protein level was determined by western blotting in LoVo and SW620 cells transfected with C miR-NC or miR-485-5p mimic, D sh-NC + anti-NC, sh-LINC01224#1 + anti-NC or anti-miR-485-5p, and E vector or MYO6 overexpression vector. Cell viability (F), number of colonies (G), apoptosis rate (H), cell rate in cell cycle phases (I, J), migration cell number (K), and invasion cell number (L) were measured by CCK-8 assay, colony formation assay, FCM and transwell assay in LoVo and SW620 cells co-transfected with miR-NC + vector and miR-485-5p mimic + vector or MYO6 overexpression vector. *P < 0.05

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