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Fig. 3 | World Journal of Surgical Oncology

Fig. 3

From: LINC01224 promotes colorectal cancer progression through targeting miR-485-5p/MYO6 axis

Fig. 3

The relationship between LINC01224 and miR-485-5p in CRC cells. A The target sites of LINC01224 and miR-485-5p. B Luciferase activity of LINC01224-WT and LINC01224-MUT vectors was measured in LoVo and SW620 cells co-transfected with miR-485-5p mimic or miR-NC. C LINC01224 expression was measured after RNA pull-down using Bio-miR-485-5p or Bio-NC. D LINC01224 and miR-485-5p levels were detected after RIP of Ago2 or IgG. E MiR-485-5p level was determined in LoVo and SW620 cells transfected with sh-NC or sh-LINC01224#1. F MiR-485-5p abundance was determined in cells with transfection of anti-miR-485-5p or anti-NC. Cell viability (G), colony formation (H), apoptosis (I), cycle process (J, K), migration (L), and invasion (M) were measured by CCK-8 assay, colony formation assay, FCM, and transwell assay in LoVo and SW620 cells co-transfected with sh-NC + anti-NC and sh-LINC01224#1 + anti-NC or anti-miR-485-5p. *P < 0.05

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