The expression of miRNA-216b is negatively correlated with 18F-FDG uptake in non-small cell lung cancer

Table 1 Real-time quantitative PCR experimental primer sequences

Gene	Sequence (5′ to 3′)
miRNA-216b	F: GCC GCG CTA AAG TGCTTA TAG TG
miRNA-216b	R: CAC CAG GGT CCG AGGT
U6	F: TGC GGG TGC TCG CTT CGG CAGC
U6	R: CCA GTG CAG GGT CCG AGGT
miRNA-145	F: CAG TCT TGT CCA GTT TTC CCAG
miRNA-145	R: TAT CCT TCT TCT CCT CTC TCT CTC
miRNA-28-3p	F: CGG ATC CAG GCC CTT CAA GGA CTT TCT
miRNA-28-3p	R: CGA ATT CAC AGA GCT CCT GCT GTG TCA

U6, miRNA-145, and miRNA-28-3p were selected as the internal reference gene when detecting the expression of miRNA-216b. Real-time quantitative PCR results were calculated using the 2^−ΔΔCT method, and each sample was repeated three times

ISSN: 1477-7819