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Table 1 Real-time quantitative PCR experimental primer sequences

From: The expression of miRNA-216b is negatively correlated with 18F-FDG uptake in non-small cell lung cancer

Gene Sequence (5′ to 3′)
miRNA-216b F: GCC GCG CTA AAG TGCTTA TAG TG
R: CAC CAG GGT CCG AGGT
U6 F: TGC GGG TGC TCG CTT CGG CAGC
R: CCA GTG CAG GGT CCG AGGT
miRNA-145 F: CAG TCT TGT CCA GTT TTC CCAG
R: TAT CCT TCT TCT CCT CTC TCT CTC
miRNA-28-3p F: CGG ATC CAG GCC CTT CAA GGA CTT TCT
R: CGA ATT CAC AGA GCT CCT GCT GTG TCA
  1. U6, miRNA-145, and miRNA-28-3p were selected as the internal reference gene when detecting the expression of miRNA-216b. Real-time quantitative PCR results were calculated using the 2−ΔΔCT method, and each sample was repeated three times