Fig. 5

KLF12 acted as the target gene of miR-876-3p. A The complementary sequences between KLF12 and miR-876-3p. B, C The interaction between miR-876-3p and KLF12 was demonstrated by dual-luciferase reporter assay and RIP assay. D, E The mRNA expression level of KLF12 in GC tissues and cells was detected by qRT-PCR assay. F The correlation between the levels of miR-876-3p and KLF12 mRNA in GC tissues was evaluated. G, H The protein level of KLF12 in GC tissues and cells was tested by western blot assay. I–K The levels of miR-876-3p and KLF12 protein in AGS and SNU-638 cells transfected with miR-NC, miR-876-3p, anti-miR-NC, or anti-miR-876-3p were detected by qRT-PCR assay and western blot assay, respectively. *P < 0.05, **P < 0.01, ***P < 0.001