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Fig. 4 | World Journal of Surgical Oncology

Fig. 4

From: Circ-RNF111 aggravates the malignancy of gastric cancer through miR-876-3p-dependent regulation of KLF12

Fig. 4

Silencing of circ-RNF111 suppressed the progression of GC cells by targeting miR-876-3p. AGS and SNU-638 cells were transfected with si-NC, si-circ-RNF111, si-circ-RNF111 + anti-miR-NC, or si-circ-RNF111 + anti-miR-876-3p. A The expression of miR-876-3p in AGS and SNU-638 cells was detected by qRT-PCR assay. B Cell viability, C colony formation, D apoptosis, E migration, and F invasion in AGS and SNU-638 cells were evaluated using CCK-8 assay, colony formation assay, flow cytometry analysis, wound-healing assay, and transwell assay, respectively. G Cell cycle process of AGS and SNU-638 cells was analyzed by flow cytometry analysis. H, I The protein levels of CyclinD1 and MMP9 in AGS and SNU-638 cells were measured by western blot assay. J–L The levels of glucose consumption, lactate production and ATP production in AGS and SNU-638 cells were examined with specific kits. M, N The protein level of HK-2 in AGS and SNU-638 cells was measured by western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001

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