Fig. 2

Knockdown of circ-RNF111 repressed the progression of GC cells. A The expression of circ-RNF111 in AGS and SNU-638 cells transfected with si-NC, si-circ-RNF111#1, or si-circ-RNF111#2 was detected by qRT-PCR assay. B The viability of si-NC, si-circ-RNF111#1, or si-circ-RNF111#2 transfected AGS and SNU-638 cells was assessed by CCK-8 assay. C–L AGS and SNU-638 cells were transfected with si-NC or si-circ-RNF111. C The colony formation, D apoptosis, E migration, and F invasion of AGS and SNU-638 cells were investigated by colony formation assay, flow cytometry analysis, wound-healing assay, and transwell assay, respectively. G, H Cell cycle process in AGS and SNU-638 cells was analyzed by flow cytometry analysis. I, J The protein levels of CyclinD1 and MMP9 in AGS and SNU-638 cells were measured by western blot assay. K–M The levels of glucose uptake, lactate production, and ATP production in AGS and SNU-638 cells were measured with commercial kits. N The protein level of HK-2 in AGS and SNU-638 cells was measured by western blot assay. **P < 0.01, ***P < 0.001