Fig. 4
From: miR-499a inhibits the proliferation and apoptosis of prostate cancer via targeting UBE2V2
miR-499a directly regulates gene expression of its target UBE2V2. A The mRNA expression levels of miR-499a in all samples and cancer cell lines. B WB analysis the expression of Cas-9 and Cas-3 protein in PC3 and LNCap cells after transfection with control miRNA, miR-499a. C Cell viability and colon formation assay after transfected with control palsmid, miR-499a plasmid, and UBE2V2 plasmid. D Cell viability and Colony formation assay of cell proliferation after PC3 cells were transfected with NC mimic, miR-499a mimic, NC inhibitor, miR-499a inhibitor, control miRNA + control plasmid, miR-499a + control plasmid, control miRNA + UBE2V2 plasmid, miR-499a + UBE2V2 plasmid. Data are shown as mean ± SEM (n = 3). Asterisks indicate significant differences from the control (*, p < 0.05; **, p < 0.01; ***, p < 0.001, Student’s t test)